Cysteine reactivity profiling identifies host regulators of <i>Mycobacterium tuberculosis</i> replication in human macrophages.

ABSTRACT: Innate immune cells such as monocytes and macrophages provide the earliest defense against infection by intracellular pathogens by initiating signaling pathways and restricting pathogen replication. However, the full complement of proteins that mediate cell-autonomous immunity remains incompletely defined. Here, we applied cysteine-directed activity-based protein profiling (ABPP) to map proteome-wide cysteine reactivity changes in THP-1 monocytes and primary human monocyte-derived macrophages during Mycobacterium tuberculosis (Mtb) infection. Across both cell types, we quantified 148 cysteine residues with altered reactivity. Genetic perturbation of a subset of proteins harboring these changes significantly impacted Mtb replication, revealing functional links between site-specific c

SUBMITTER: Neff J 

PROVIDER: S-EPMC12424734 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature