Human-mouse cross-species comparison identifies common and unique aspects of intestinal mesenchyme development.

ABSTRACT: Using single-cell RNA sequencing (scRNA-seq) and histological approaches, we examine cross-species cellular identity, diversity and organization of mesenchymal (fibroblast) populations in the developing mouse and human intestines. In both species, we defined 7 fibroblast populations. Using cross-species integration and label transfer approaches we find that each mesenchymal cell subtype in the murine intestine is highly concordant to a cell type/state in the human intestine, and vice-versa, suggesting a strong conservation of mesenchymal cell types/states across species. Despite this conservation, we also observe that individual lineage-defining genes are not always shared and can be found in different mesenchymal populations. High resolution spatial analysis via fluorescent in situ hybrid

SUBMITTER: Johnson K 

PROVIDER: S-EPMC12424763 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature