4-Pi Stimulated Raman Scattering for Label-free Super-resolution Chemical Imaging.

ABSTRACT: Super-resolution fluorescence microscopy has transformed the study of biological structures and functions beyond the diffraction limit. Unlike fluorescence methods, label-free chemical imaging, mostly based on Raman and infrared spectroscopy, provides intrinsic molecular contrast, enabling the study of biomolecules, nanostructures, drug molecules, and metabolites that cannot be easily tagged. However, while a wide range of fluorescence-based super-resolution techniques are well-established, extending super-resolution to label-free chemical imaging has remained challenging due to low signal levels and limited resolution improvement. Super-resolution stimulated Raman scattering microscopy (SRS) is most promising due to its high sensitivity and imaging speed. Similar to fluorescence, existing

SUBMITTER: Kim JI 

PROVIDER: S-EPMC12424818 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature