The Glc7/PP1 phosphatase triggers Paf1C dissociation from RNA polymerase II to enable transcription termination.

ABSTRACT: The mechanisms that control the dynamic composition of RNAPII elongation complexes govern major transitions in the transcription cycle yet are poorly understood. Here, we show that the transcription elongation factor Spt5 determines elongation complex composition to promote productive elongation and the transition to termination. Using an unbiased genetic screen and genomic approaches in Saccharomyces cerevisiae, we provide evidence that dephosphorylation of the Spt5 C-terminal repeat domain (CTR) by the Glc7/PP1 phosphatase is required to dislodge Paf1C from RNAPII near the cleavage and polyadenylation site (CPS). Mutations in Paf1C or the Spt5 CTR that dissociate Paf1C from RNAPII bypass the requirement for two critical regulatory subunits of Glc7. Depletion of Glc7 causes aberran

SUBMITTER: Namjilsuren S 

PROVIDER: S-EPMC12424955 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature