Mitogen- and stress-activated kinase 1 in primary sensory neurons contributes to formalin-induced tonic pain.

ABSTRACT:

Introduction

The nuclear mitogen- and stress-activated kinases (MSKs) play a critical role in the development and persistence of pain after tissue injury.

Objectives

Here, we ascertained the MSK isoform, the cells and mechanisms, which mediate MSKs' pronociceptive function.

Methods

Nocifensive behaviour evoked by subcutaneous formalin injection into the paw was quantified in wild type (WT), MSK1 and MSK2 global knock out (MSK1^-/- and MSK2^-/-) mice, and a month after injecting adeno-associated viral vector carrying short-hairpin (sh) RNA directed towards the MSK1-encoding gene Rps6ka5 mRNA or scrambled shRNA into the sciatic nerve of WT mice. Rps6ka5 expression in nociceptors was ascertained by analysing publicly available single cel

SUBMITTER: Irfan J 

PROVIDER: S-EPMC12425037 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature