A Novel Raman-Chromatography Assembly for Automated Calibration and In-Line Monitoring in Bioprocessing.

ABSTRACT: Optical spectroscopic techniques have been successfully employed in bioprocessing as process analytical technology for real-time process monitoring in numerous applications. The implementation of spectroscopy-based PAT techniques commonly necessitates the generation of representative process data used for calibration and validation of multivariate statistical models for analyzing the sample composition in real-time. To automate the generation of such data, we present a novel assembly of a commercially available chromatography system in combination with a Raman spectrometer for fast and accurate acquisition of Raman spectra. Using the ultra-/diafiltration (UF/DF) process as a case study, our methodology involved the preparation of representative calibration and validation mixtures of phosph

SUBMITTER: Heyer-Muller J 

PROVIDER: S-EPMC12425122 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature