Adenoviral vectors can impair adrenocortical steroidogenesis: clinical implications for natural infections and gene therapy.

ABSTRACT: Recombinant adenoviral vectors are effective in transferring foreign genes to a variety of cells and tissue types, both in vitro and in vivo. However, during the gene transfer, they may alter the principal function and local environment of transfected cells. Increasing evidence exists for a selective adrenotropism of adenovirus during infections and gene transfer. Therefore, using bovine adrenocortical cells in primary culture, we analyzed the influence of different adenoviral deletion mutants on cell morphology and physiology. Transfection of cells with an E1/E3-deleted adenoviral vector, engineered to express a modified form of the Aequorea victoria green fluorescent protein, was highly efficient, as documented by fluorescent microscopy. Ultrastructural analysis, however, demonstrated nu

SUBMITTER: Alesci S 

PROVIDER: S-EPMC124257 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature