The expression regulation of <i>recA</i> gene and bacterial class 2 integron-associated genes induced by antibiotics.

ABSTRACT:

Objective

To investigate the effects and mechanisms of common antibiotics induction on the expression of class 2 integron integrase and variable region resistance genes in bacteria, as well as potential structural mutations.

Methods

Clinical isolates containing non-functional class 2 integrons and functional class 2 integrons were selected. Strains containing non-functional class 2 integrons or functional class 2 integrons were constructed using isolated DNA templates. These strains were subjected to continuous induction with drug concentrations of 1/2 MIC and 1/4 MIC (ciprofloxacin, ampicillin, and kanamycin) and a concentration of 0.2 μg/ml (mitomycin C) over 8 days. The relative expression levels of relevant genes were measured on days 1, 3, and 8. Drug resistance in the

SUBMITTER: Ye J 

PROVIDER: S-EPMC12425958 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature