High-glucose diets differentially modulate phosphatidylcholine metabolism and fecundity in <i>Caenorhabditis elegans</i>.

ABSTRACT:

Background

Caenorhabditis elegans fed a high-glucose Escherichia coli OP50 diet exhibit reduced fecundity, but the underlying mechanisms remain unclear.

Methods

A differential high-glucose diet paradigm was established using C. elegans fed two bacterial diets that produced distinct fecundity outcomes under high-glucose conditions. The effects of these diets in varying conditions were analyzed through transcriptomic, lipidomic, and metabolomic profiling to correlate with fecundity. Supplementation experiments were further performed to validate the links between changes in lipid metabolism and fecundity. By characterizing the gerlime phenotypes, we constructed a model to interpret how dietary inputs alter oogenesis signaling and, consequently, fecundity outcomes.

SUBMITTER: Wang CW 

PROVIDER: S-EPMC12425989 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature