Ancestral genomic functional differences in oligodendroglia: implications for Alzheimer's disease.

ABSTRACT:

Introduction

This study investigates ancestry-specific changes in induced pluripotent stem cell (iPSC)-derived oligodendroglia genomic regulation in Alzheimer's disease (AD), addressing diversity gaps by including African, Amerindian, and European ancestries in the analysis.

Methods

We generated 12 iPSC lines from AD patients and controls with different apolipoprotein E (APOE) genotypes, APOE ε3/ ε3 and APOE ε4/ ε4, across three ancestries. Lines were differentiated into neural spheroids containing oligodendrocyte lineage cells and analyzed by single-nucleus RNA sequencing, Assay for Transposase-Accessible Chromatin with sequencing (ATACseq)APO, and High-throughput Chromosome Conformation Capture (Hi-C).

Results

We identified ancestry-specific differences in gene expr

SUBMITTER: Ramirez AM 

PROVIDER: S-EPMC12426912 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature