Fingerprinting the Intestinal Transport of Low-Molecular-Mass Advanced Glycation End-Products (AGEs) Using a Caco-2 Transwell Model.

ABSTRACT: Food-borne advanced glycation end-products could potentially contribute to the endogenous AGE accumulation within the body, albeit to a different extent for different AGEs. This study focuses on characterizing intestinal absorption and intracellular accumulation of 10 selected free low-molecular-mass (LMM) advanced glycation end-products (AGEs) to obtain insight into potential differences in their systemic bioavailability by using a Caco-2 transwell model. The findings reveal that all tested AGEs can cross the intestinal barrier through the paracellular route, albeit to a limited extent. Glycolic acid-lysine-amide (GALA) shows the highest transport percentage, reaching 1.1% ± 0.3% after 2 h, while N-ε-(carboxymethyl)lysine (CML) displays the highest level of accumulation in intestinal cell

SUBMITTER: Li X 

PROVIDER: S-EPMC12426922 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature