A "Steady-State" Relaxation Dispersion Nuclear Magnetic Resonance Experiment for Studies of Chemical Exchange in Degenerate ¹H Transitions of Methyl Groups.

ABSTRACT: Degenerate spin-systems consisting of magnetically equivalent nuclear spins, such as a ¹H₃ spin-system in selectively ¹³CH₃-labeled proteins, present considerable challenges for the design of Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion NMR experiments to characterize chemical exchange on the micro-to-millisecond time-scale. Several approaches have been previously proposed for the elimination of deleterious artifacts observed in methyl ¹H CPMG relaxation dispersion profiles obtained for (¹³C)¹H₃ groups. We describe an alternative, experimentally simple solution and design a "steady-state" methyl ¹H CPMG scheme, where 90° or acute-angle (<90°) ¹H radiofrequency pulses are

SUBMITTER: Tugarinov V 

PROVIDER: S-EPMC12427130 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature