Survivin Is a Central Mediator of Cell Proliferation in HPV-Negative Head and Neck Squamous Cell Carcinoma.

ABSTRACT:

Background/objectives

HNSCC is a highly aggressive malignancy marked by the dysregulation of the cell cycle. In HPV^- HNSCC, mutations in the CDKN2A gene frequently result in the loss of the p16 protein, a key inhibitor of the cyclin D1/CDK4/6 complex. This loss results in unchecked G1/S phase progression. The CDK4/6 inhibitor palbociclib has shown therapeutic potential in HPV^- HNSCC by inducing G1 phase arrest and reducing cell viability. In this study, we investigated the molecular mechanisms by which palbociclib affects cell viability in HPV^- HNSCC.

Methods

Four HPV^- HNSCC cell lines were treated with palbociclib, and RNA sequencing was performed to assess changes in gene expression. Cell viability was measured using the MTT assa

SUBMITTER: Zhu J 

PROVIDER: S-EPMC12427275 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature