Development and Validation of Tetranucleotide Repeat Microsatellite Markers at the Whole-Genome Level in the Yangtze Finless Porpoise.

ABSTRACT: The Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis, YFP) is the only freshwater cetacean species currently found in China's Yangtze River. To accurately evaluate its genetic diversity and provide reliable molecular markers for population genetic studies, this study developed a highly efficient and reproducible method for identifying polymorphic microsatellite loci using whole-genome sequencing data. Using this method, we identified and validated a set of highly polymorphic microsatellite markers, which were then used to analyze the genetic diversity of the YFP populations in Poyang Lake to evaluate their effectiveness. Our results demonstrated that the screening pipeline successfully identified 220 tetranucleotide repeat microsatellite loci. Based on the princ

SUBMITTER: Tang M 

PROVIDER: S-EPMC12427282 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature