Time- and Dose-Dependent Effects of Irradiation on Endothelial and Tumor Endothelial Cells: Transcriptional, Molecular, and Functional Changes Driving Activation In Vitro and In Vivo.

ABSTRACT: Background: Irradiation (IR) targets cancer cells, but also the tumor microenvironment, including the tumor's blood vessels. In addition to tumor endothelial cell (TEC) apoptosis, IR can lead to TEC activation, potentially increasing immune cell infiltration. However, the changes underlying the IR-induced activation of endothelial cells (ECs) are poorly understood. This study investigated dose- and time-dependent molecular and functional responses of murine and human EC lines to IR in vitro and TECs in vivo in murine tumor models of colorectal carcinoma. Methods: HUVEC, EA.hy926, and Hulec5a, as well as murine bEND.3, 2H11, and SVEC4-10 EC lines, were irradiated with single doses of 2-10 Gy. EC proliferation and survival after IR were assessed by staining all nuclei (Hoechst

SUBMITTER: Santek I 

PROVIDER: S-EPMC12427429 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature