Topographic cues regulate collective cell dynamics in curved nano/microgrooved tubular microchannels.

ABSTRACT: Physical properties of the extracellular matrix, such as topography and curvature, regulate collective epithelial behaviors. However, the interplay between these geometric factors on collective migration is not well understood. In this study, we investigate the effects of topographic cues on a curved surface on collective epithelial migration within tubular microchannels with an inner diameter of 100 μm. These tubular microchannels feature circumferential or longitudinal micro- and nano-grooves fabricated by two-photon polymerization three-dimensional printing and micro-molding techniques. Live cell microscopy records the collective migration of GFP-labeled epithelial cells into the microchannel with each topographical design. We utilized a single-cell behavior analysis for the tracked tim

SUBMITTER: Matsubara T 

PROVIDER: S-EPMC12427609 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature