Identification of the Centrifugation-Caused Paralytic Impact on Neutrophils.

ABSTRACT: To investigate granulocytes under laboratory conditions, centrifugation steps are typically required for the isolation of neutrophil granulocytes from whole blood. However, only a few studies to date have addressed the direct effects of centrifugation itself on the functional state of neutrophils. This study aims to elucidate the mechanisms that contribute to the modification of granulocytes during centrifugation. We hypothesize that granules sustain morphological alterations during centrifugation, leading to the release of highly potent antimicrobial enzymes into the cytosol of the cells. Neutrophils were isolated from whole blood using different methods with and without centrifugation and analyzed by flow cytometry, ELISA, and mass spectrometry. Our findings demonstrate that intracellula

SUBMITTER: Pehl S 

PROVIDER: S-EPMC12427856 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature