Development of an colloidal gold immunochromatography assay strip for the diagnosis of <i>Babesia canis</i>.

ABSTRACT:

Introduction

Canine babesiosis, caused by Babesia canis, is a tick-borne hemolytic disease requiring rapid, reliable diagnostic tools to protect canine health in resource-limited settings.

Methods

We developed a colloidal gold immunochromatographic assay (CGIA) strip using a recombinant BcMSA1-BcSA1 fusion protein, expressed in Escherichia coli with a yield of 2.5 mg/L, combining hydrophilic domains of merozoite surface antigen (BcMSA1) and secreted antigen (BcSA1). Seventy-two serum samples from veterinary clinics in Shanxi Province, China (ethical approval SXAU-2022-013), were tested against a commercial ELISA kit (Anigen Rapid B. canis Ab Test Kit), with infections confirmed by microscopy and PCR. Specificity was assessed using sera positive for Theileria spp., Toxoplasma

SUBMITTER: Wang J 

PROVIDER: S-EPMC12428028 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature