Integrated Transcriptomic and Proteomic Analyses Revealed the Mechanism of the Osmotic Stress Response in <i>Lacticaseibacillus rhamnosus</i> ATCC 53103.

ABSTRACT: Lacticaseibacillus rhamnosus (Lbs. rhamnosus) is renowned for its tolerance to gastric acid and adaptability to bile and alkaline conditions, and is crucial for intestinal health and immune regulation. In this study, integrated transcriptomic and proteomic analyses were employed to elucidate the response mechanisms of Lbs. rhamnosus under osmotic stress, induced by exposure to 0.6 M sodium lactate, which elevates environmental osmotic pressure. It was shown that 792 differentially expressed genes and 138 differentially expressed proteins were detected in Lbs. rhamnosus ATCC 53103 treated with osmotic stress. The differential regulation of these genes/proteins mainly includes the inhibition of fatty acid metabolism with membrane structural remodeling (downregulat

SUBMITTER: Luo W 

PROVIDER: S-EPMC12428430 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature