Interactions of <i>Galleria mellonella</i> Proline-Rich Antimicrobial Peptides with Gram-Negative and Gram-Positive Bacteria.

ABSTRACT: Two proline-rich antimicrobial peptides (PrAMPs), named P1 and P2, purified from hemolymph of the greater wax moth Galleria mellonella, were studied for their effects on Gram-negative (Escherichia coli) and Gram-positive (Micrococcus luteus) bacteria. Both peptides decreased the M. luteus bacterial survival rate and caused E. coli bacterial membrane permeabilization. However, in both cases, the P2 peptide was approximately three times more effective than the P1 peptide. Fluorescence microscopy imaging demonstrated binding of both FITC-labeled peptides to E. coli and M. luteus cells. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) imaging of peptide-treated bacteria revealed considerable changes in cell morphology, cell s

SUBMITTER: Zdybicka-Barabas A 

PROVIDER: S-EPMC12428502 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature