Deficiency of N1-Adenine Methyltransferase Aggravates RNA and Protein Aggregation.

ABSTRACT: RNA modifications regulate diverse aspects of transcripts' function and stability. Among these, N1-methyladenine (m¹A) is a reversible mark primarily installed by the TRMT6/TRMT61A methyltransferase on tRNA, though it is also found on other RNA types. m¹A has been implicated in protecting mRNAs during acute protein misfolding stress. However, the role of m¹A under chronic proteotoxic conditions, such as intracellular amyloid aggregation, remains poorly understood. To address this gap, we examined the effects of reduced N1-adenine methylation in human cells undergoing amyloidogenesis. Suppression of the methyltransferase TRMT61A or overexpression of the m¹A-specific demethylase ALKBH3 enhanced amyloid aggregation. A deficiency of N1-adenine methyl

SUBMITTER: Alriquet M 

PROVIDER: S-EPMC12428715 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature