UHPLC-MS/MS-Based Metabolomics Identifies Freshness Biomarkers and Temporal Spoilage Threshold in Refrigerated Goose Meat.

ABSTRACT: The dynamic metabolic landscape underlying goose meat quality deterioration during refrigerated storage remains incompletely elucidated. Here, ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based widely targeted metabolomics was employed to characterize metabolic profiling in refrigerated goose meat. Orthogonal partial least squares discriminant analysis (OPLS-DA) revealed 211 differential metabolites, while random forest regression (RFR) identified 30 candidate biomarkers. Seven metabolites, including xanthine, oxidized glutathione, and inosine 5'-monophosphate, exhibited significant correlations with total volatile basic nitrogen (TVB-N). By integrating potential biomarkers, metabolic pathways involving purines, amino acids, and sugars were identified

SUBMITTER: Gao W 

PROVIDER: S-EPMC12428737 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature