Acute Toxicity of Metal Oxide Nanoparticles-Role of Intracellular Localization In Vitro in Lung Epithelial Cells.

ABSTRACT: Endocytic uptake and lysosomal localization are suggested to be the key mechanisms underlying the toxicity of metal oxide nanoparticles (MONPs), with dissolution in the acidic milieu driving the response. In this study, we aimed to investigate if MONPs of varying solubility are similarly sequestered intracellularly, including in lysosomes and the role of the acidic lysosomal milieu on toxicity induced by copper oxide (CuO) nanoparticles (NPs), nickel oxide (NiO) NPs, aluminum oxide (Al₂O₃) NPs, and titanium dioxide (TiO₂) NPs of varying solubility in FE1 lung epithelial cells. Mitsui-7 multi-walled carbon nanotubes (MWCNTs) served as contrasts against particles. Enhanced darkfield hyperspectral imaging (EDF-HSI) with fluorescence microscopy was used to dete

SUBMITTER: Boyadzhiev A 

PROVIDER: S-EPMC12428783 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature