Anandamide Alters Glycolytic Activity in <i>Streptococcus mutans</i>: Metabolomics and Stable Isotope Labeling Study.

ABSTRACT: Streptococcus mutans (S. mutans) is a cariogenic bacterium in the oral cavity that plays a significant role in plaque formation and dental caries. In previous research by our group, we showed that the endocannabinoid anandamide (AEA) has anti-bacterial and anti-biofilm activities against S. mutans. Here, we aimed to investigate its effects on S. mutans through metabolomics analyses. S. mutans was cultivated in the absence or presence of AEA at a sub-minimum inhibitory concentration (MIC), and changes in metabolites and metabolic pathways were assessed through liquid chromatography-mass spectrometry (LC-MS). Treatment of S. mutans using AEA at 10 µg/mL significantly disturbed the glycolytic flux in the bacteria, which was indicated by a reduced glucose upt

SUBMITTER: Wolfson G 

PROVIDER: S-EPMC12429060 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature