Comprehensive Characterisation of Extracellular Vesicle Preparations Using Multiparametric Size-Exclusion Chromatography.

ABSTRACT: Extracellular vesicles (EVs) hold great promise in the fields of diagnostics and therapeutics. However, the heterogeneity of these membrane-enclosed messengers and the complexity of the biological samples in which they occur pose significant research challenges. The aim of this study was to improve the reliability of size-exclusion chromatography (SEC) and immunolabelling as common approaches in the EV field, and to provide a comprehensive characterisation tool for diverse EV preparations. Profiling of SEC-separated sample components was conducted through light absorbance, fluorescence, and light scattering, providing insights into particle content, size, protein content, and specific markers. Key considerations for assay robustness, including SEC mobile phase composition and immunostainin

SUBMITTER: Bozic D 

PROVIDER: S-EPMC12429111 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature