Four Large Indels in Barley Chloroplast Mutator (<i>cpm</i>) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System.

ABSTRACT: A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNA^His and rps19 genes, a 620 bp deletion in the psbA gene, a 79 bp deletion in the intergenic region between rpl33 and rps18 genes and a 45 bp deletion in the rps3 gene. The present investigation aims to understand the mechanisms producing the large indels and to better characterize the cpm mutagenic effect. Whole plastome sequencing revealed novel polymorphisms that were identified either in regions not previously examined or in regions

SUBMITTER: Lencina F 

PROVIDER: S-EPMC12429641 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature