Optimizing Tissue Sampling Timing for Accurate Gene Expression Analysis.

ABSTRACT: The reliability of molecular diagnostic and prognostic tools is contingent on the quality of biospecimens, which are often collected during surgical procedures. This study investigated the impact of surgical manipulation on gene expression in the urinary bladder mucosa during radical cystectomy. Seventeen patients with urinary bladder cancer were enrolled, and paired pre- and post-surgery biopsies were analyzed. Pre-surgical biopsies were obtained in situ under anesthesia, while post-surgical biopsies were collected ex vivo following bladder removal. Total RNA was extracted, and gene expression was assessed using qPCR arrays, measuring the expression of 374 inflammation-related genes. The findings from the exploratory phase were further validated by analyzing key genes in an independent pa

SUBMITTER: Davidsson S 

PROVIDER: S-EPMC12429838 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature