Decoding transcriptional identity during neuron-astroglia cell fate driven by RAR-specific agonists.

ABSTRACT: How cells respond to different signals leading to defined lineages is an open question to understand physiological differentiation leading to the formation of organs and tissues. Among the various morphogens, retinoic acid signaling, via the RXR/RAR nuclear receptors activation, is a key morphogen of nervous system development and brain homeostasis. Here, we analyze gene expression in ∼80 000 cells covering 16 days of monolayer mouse stem cell differentiation driven by the pan-RAR agonist all-trans retinoic acid, the RARα agonist BMS753 or the activation of both RARβ and RARγ receptors (BMS641 + BMS961). Furthermore, we have elucidated the role of these retinoids for driving nervous tissue formation within 90 days of brain organoid cultures, by analyzing >8000 distinct spatial regions over

SUBMITTER: Galindo-Albarran A 

PROVIDER: S-EPMC12430019 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature