Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in <i>Brassica napus</i> L. and Functional Characterization of <i>BnBGLU77</i>.

ABSTRACT: The β-glucosidases (BGLUs) of Glycoside Hydrolase Family 1 (GH1) exhibit essential functions in plant secondary metabolism and stress responses, mediated by their dual catalytic capabilities in hydrolysis and transglycosylation. This study identified 149 BGLU family members within B. napus (Brassica napus L.), which were systematically categorized into 10 distinct subgroups. Subsequent characterization encompassed detailed examination of their motif composition, chromosomal distribution, gene collinearity, selection pressure, and expression profiling. Transient overexpression of BnBGLU77 in N. benthamiana (Nicotiana benthamiana), combined with untargeted metabolomics analysis, revealed pronounced modulatory effects on the degradation and accumulation of β

SUBMITTER: Wei X 

PROVIDER: S-EPMC12430181 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature