Integrated Histological, Ultrastructural, and Transcriptomic Analyses Reveal New Insights into Stamen Development in Cytoplasmic Male Sterile Tobacco (CMS K326).

ABSTRACT: Cytoplasmic male sterility (CMS) is a crucial tool for exploiting plant heterosis, though its underlying mechanisms in tobacco remain incompletely understood. In this study, Tobacco CMSK326, derived from a naturally occurring variant of Nicotiana tabacum, exhibited a range of stamen abnormalities, including stamen degeneration, stamen absence, and carpelloid, petaloid, and sepaloid traits. Histological and electron microscopy showed that CMS K326 and its maintainer differentiated at the early bud stage. Analysis of differentially expressed genes (DEGs) revealed abnormal expressions of several key genes, including WUSCHEL (WUS), GLOBOSA (GLO), SUPERMAN (SUP), and auxin-related genes such as AUX22, during bud development. Weighted gene

SUBMITTER: Cui F 

PROVIDER: S-EPMC12430244 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature