A Genome-Wide Modeling and Characterization Study of Pleckstrin Homology Domains in <i>Chlamydomonas reinhardtii</i>.

ABSTRACT: The function of pleckstrin homology (PH) domains is to recognize and bind to specific phosphoinositides in the membranes as part of diverse cellular signaling processes. The structure of some PH domains has been solved by X-ray crystallography, but structures of many PH domains remain to be elucidated. In green alga Chlamydomonas reinhardtii, none of the PH domains have been crystallized or characterized. The goal of our study was to model and characterize in detail the structures of all eleven of the PH domains identified in C. reinhardtii. Our computational strategy of integrating the information available on sequence, structure, and function with modeling and biophysical characterization has uncovered new biological predictions for these proteins. These predictions can be

SUBMITTER: Aksoy M 

PROVIDER: S-EPMC12430284 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature