Pectin Peek-a-Boo: Homogalacturonan Turnover During Flooding-Induced Legume Root Aerenchyma Formation.

ABSTRACT: Flooding can cause root hypoxia and can lead to significant agricultural losses. Therefore, understanding plant adaptations to flooding, including root aerenchyma development, is one important avenue for insuring future global food security. We investigated cell wall modifications during root aerenchyma formation in response to the prolonged 0-48 h flooding of Phaseolus coccineus, Pisum sativum, and Cicer arietinum seedlings. Using transmission electron microscopy, toluidine blue O (TBO) staining, and immunolabeling with antibodies targeting de-methyl-esterified homogalacturonan (DMEH), partially DMEH, and methyl-esterified homogalacturonan (MEH), we examined changes in cell wall composition. Transmission electron microscopy and TBO staining revealed degradation of cel

SUBMITTER: Pegg TJ 

PROVIDER: S-EPMC12430375 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature