Individual and Combined Effects of Medium- and Long-Chain Triacylglycerol and 2'-Fucosyllactose on Small Intestinal Morphology, Barrier Function, and Gut Microbiota in Growing C57BL/6 Mice.

ABSTRACT: Background/Objectives: Medium- and long-chain triacylglycerol (MLCT) and 2'-fucosyllactose (2'-FL) are functional ingredients abundant in human milk; however, their effects on small intestinal development and health remain largely unknown, and no research has explored their potential combined effects. Methods: In this study, growing C57BL/6 mice (3 weeks old) were fed diets without or with 2.5 g/100 g of MLCT, 2'-FL, or the combination (MLCT + 2'-FL; 5:1) for 21 days. Body weight, major organ indices, small intestinal morphology-related indicators (small intestinal length, villus height, crypt depth, villus height/crypt depth (V/C) ratio, and epithelial cell proliferation), and intestinal barrier function markers (goblet cell and Paneth cell count, protein expression of ZO-1

SUBMITTER: Jin X 

PROVIDER: S-EPMC12430467 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature