Direct Analysis of Solid-Phase Carbohydrate Polymers by Infrared Multiphoton Dissociation Reaction Combined with Synchrotron Radiation Infrared Microscopy and Electrospray Ionization Mass Spectrometry.

ABSTRACT: To determine the structure of carbohydrate polymers using conventional analytical technology, several complicated steps are required. We instead adopted a direct approach without the need for pretreatments, using an intense infrared (IR) laser for carbohydrate analysis. IR free-electron lasers (FELs) driven by a linear accelerator possess unique spectroscopic features, including extensive wavelength tunability and high laser energy in the IR region from 1000 cm^-1 (10 μm) to 4000 cm^-1 (2.5 μm). FELs can induce IR multiphoton dissociation reactions against various molecules by supplying vibrational excitation energy to the corresponding chemical bonds. Chitin from crayfish and cellulose fiber were irradiated by FELs tuned to νC-O (9.1-9.8 μm), νC-H (3.5 μm), and δH-C-O

SUBMITTER: Kawasaki T 

PROVIDER: S-EPMC12431057 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature