Pbs2 regulates late-stage macroautophagy in Saccharomyces cerevisiae.

ABSTRACT: Autophagy is crucial for maintaining cellular homeostasis and is linked to various diseases. In Saccharomyces cerevisiae, the Polymyxin B Sensitivity 2 (Pbs2) protein is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in mitophagy. To explore the potential role of Pbs2 in macroautophagy, we engineered wild-type and PBS2-deficient cells using plasmid construction and yeast transformation techniques, followed by a series of autophagy assays. First, after nitrogen starvation, the levels of autophagic activity were evaluated with the classical GFP-Atg8 cleavage assay and the Pho8Δ60 activity assay at different time points. Deleting PBS2 significantly decreased both GFP-Atg8 protein cleavage and Pho8Δ60 activity, indicating that Pbs2 is essential for macroautopha

SUBMITTER: Song J 

PROVIDER: S-EPMC12431567 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature