Metabolic alterations in human pulmonary artery smooth muscle cells treated with PDGF-BB.

ABSTRACT:

Background

Metabolic abnormalities are considered to play a key regulatory role in vascular remodeling of pulmonary arterial hypertension. However, to date, there is a paucity of research documenting the changes in metabolome profiles within the supernatants of pulmonary artery smooth muscle cells (PASMC) during their transition from a contractile to a synthetic phenotype.

Methods

CCK-8 and Edu staining assays were used to evaluate the cell viability and proliferation of human PASMCs. IncuCyte ZOOM imaging system was used to continuously and automatically detect the migration of the PASMCs. A targeted metabolomics profiling was performed to quantitatively analyze 121 metabolites in the supernatant. Orthogonal partial least squares discriminant analysis was used to discrimina

SUBMITTER: Zhang MJ 

PROVIDER: S-EPMC12431571 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature