Green-to-red spectral labeling: A novel polysynaptic retrograde tracing strategy in the marker footprint mouse model.

ABSTRACT:

Background

Rabies virus (RABV)-derived neuronal tracing tools are extensively applied in retrograde tracing due to their strict retrograde transsynaptic transfer property and low neurotoxicity. However, the RABV infection and expression of fluorescence products would be gradually cleared while the infected neurons still survive, a phenomenon known as non-cytolytic immune clearance (NCLIC). This phenomenon introduced the risk of fluorescence loss and led to the omission of a subset of neurons that should be labeled, thereby interfering in the analysis of tracing results.

Methods

To compensate for the fluorescence loss problem, in this study, we developed a novel marker footprints (MF) mouse, involving a Cre recombinase-dependent red fluorescent reporter system and systemic ex

SUBMITTER: Song Y 

PROVIDER: S-EPMC12431593 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature