Spatial and longitudinal tracking of enhancer-AAV vectors that target transgene expression to injured mouse myocardium.

ABSTRACT: Tissue regeneration enhancer elements (TREEs) direct expression of target genes in injured and regenerating tissues. Additionally, TREEs of zebrafish origin were shown to direct expression of transgenes in border zone regions after cardiac injury when packaged into recombinant adeno-associated viral (AAV) vectors and introduced into mice. Future implementation of TREEs into AAV-based vectors as research tools and potential gene therapy modalities requires a deeper understanding of expression dynamics and potential off-target effects. Here, we applied in vivo bioluminescent imaging to mice systemically injected with AAV vectors containing different combinations of capsids, enhancers, and timing of delivery. Longitudinal tracking of expression directed by different TREEs revealed distinct am

SUBMITTER: Wolfson DW 

PROVIDER: S-EPMC12431772 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature