Validation and use of the dead-end ultrafiltration method for the capture and recovery of Shiga toxin-producing Escherichia coli from surface water.

ABSTRACT: Root cause analysis following microbial water contamination events typically relies upon the collection of large sample volumes. Dead-end ultrafiltration (DEUF) is an efficient method for sampling and transporting up to 100 L of water from field environments. A single-lab validation (SLV) using DEUF to recover Shiga toxin-producing Escherichia coli (STEC) was recently completed using 100 L surface water samples ranging in turbidity from 3.7 to 12.8 NTU (nephelometric turbidity unit). The SLV trials using E. coli O157:H7 and O121:H19 indicated that a positive recovery rate (25%-85%) from eluates was achievable after inoculating ultrafilters with low-level contamination concentrations of ≤6 CFU/filter (where CFU is colony forming unit). Higher inoculum levels, >27 CFU/filter, resulted in pos

SUBMITTER: Kataoka A 

PROVIDER: S-EPMC12431952 | biostudies-literature | 2025 Sep-Oct

REPOSITORIES: biostudies-literature