Single-cell RNA sequencing reveals developmental trajectories and environmental regulation of callus formation in Arabidopsis.

ABSTRACT: Plant cells exhibit an extraordinary regenerative potential, achieving cellular totipotency by dedifferentiating to form new tissues. While significant progress has been made in understanding cell fate mechanisms, the regulatory networks governing callus cell development remain insufficiently explored, particularly regarding cell classification, morphology, and regulatory processes. This study provides a detailed investigation into the developmental dynamics and transcriptomic profiles of callus cells in Arabidopsis at key stages: initiation, proliferation, and greening. Employing single-cell RNA sequencing and UMAP-based clustering, we annotated cell clusters based on highly enriched gene expressions. Developmental trajectories were further mapped through pseudotime analysis, revealing di

SUBMITTER: Liu Z 

PROVIDER: S-EPMC12431987 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature