Lab-on-PCB with integrated DNA amplification and electroanalytical detection for point-of-care diagnostics.

ABSTRACT: Nucleic acid amplification tests (NAATs) are powerful medical diagnostic tools for point-of-care (POC) and other field applications. However, traditional methods like quantitative PCR (qPCR) require complex, expensive equipment and trained operators, limiting their use to centralized labs. Isothermal alternatives, like Loop-mediated Isothermal Amplification (LAMP), are better adapted for POC devices. Lab-on-PCB systems have the potential to overcome the challenges faced by conventional microfabrication-based systems. This study presents a novel lab-on-PCB device for nucleic acid amplification and electrochemical detection using reverse transcription LAMP (RT-LAMP) of SARS-CoV-2. The system consists of two disposable PCB-based chips making it close to zero cost. One PCB is for heating and n

SUBMITTER: Thorapalli Muralidharan S 

PROVIDER: S-EPMC12432110 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature