Not only an inhibitor: Trehalose enhances the catalytic action exerted on oxaloacetate by rabbit lactate dehydrogenase.

ABSTRACT: Trehalose is an osmolyte featuring a prominent competence in stabilizing proteins and enzymes. In particular, in the presence of this peculiar disaccharide, quite a number of enzymes show improved stability against thermal denaturation and an enhanced ability to withstand exposure to freezing-thawing cycles. Moreover, it was recently reported that trehalose counteracts the acid-induced dissociation of oligomeric protein complexes. Concerning the catalytic action of enzymes, the addition of trehalose to assay mixtures was found to decrease the values of both K_m and k_cat, with the decrease in reaction velocity related to the increase in viscosity induced by the disaccharide. Here, we show that trehalose does not necessarily perform as a negative effector on reaction vel

SUBMITTER: Stefan A 

PROVIDER: S-EPMC12432435 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature