Overexpression of miR-1283 inhibits cell proliferation and migration of colorectal cancer cells by targeting PDZD8.

ABSTRACT:

Background

PDZ domain-containing protein 8 (PDZD8) has been implicated in the progression of colorectal cancer. However, its regulatory mechanisms and functional impact remain to be fully elucidated. We assessed PDZD8 expression in colorectal cancer cell lines and normal colorectal mucosa using Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Methods

The role of PDZD8 in colorectal cancer was further investigated through the generation of PDZD8-depleted HCT 116 and RKO cell models via short hairpin RNA (shRNA) interference. The influence of PDZD8 knockdown on cellular phenotypes was examined through proliferation, migration, apoptosis, and cell cycle assays. Additionally, the regulatory effect of miR-1283 on PDZD8 was explored both in vitr

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12432640 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature