Decoding hub gene networks and miRNA interplay in Wilms tumor pathogenesis and therapeutic sensitivity.

ABSTRACT:

Objectives

This study aims to explore the expression and functional significance of hub genes in Wilms tumors and their potential as diagnostic biomarkers and therapeutic targets.

Methods

Gene expression data from Wilms tumors and normal control samples were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using the limma package in R, followed by Venn diagram analysis to identify common DEGs. STRING and Cytoscape were employed to construct a protein-protein interaction (PPI) network and identify hub genes. Cell culture of five Wilms tumor cell lines and normal controls was performed to validate gene expression. Functional assays including proliferation, colony formation, and wound healing assays were conducted t

SUBMITTER: Peng W 

PROVIDER: S-EPMC12432689 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature