Mechanistic insights into the anti-tumor effects of neochlorogenic acid in hepatocellular carcinoma: <i>in vitro</i> and <i>in vivo</i> studies.

ABSTRACT:

Background

Neochlorogenic acid (NCA), a naturally occurring polyphenolic compound, exhibits diverse biological activities. This study aimed to investigate the inhibitory effects of NCA on hepatocellular carcinoma (HCC) cells and elucidate its underlying molecular mechanisms.

Methods

The anti-proliferative activity of NCA on human HCC cell lines HepG2 and Huh-7 was assessed using the Cell Counting Kit-8 (CCK-8) assay. Flow cytometry was employed to analyze apoptosis and cell cycle distribution. Wound-healing assays were conducted to evaluate the effects of NCA on cell migration. Transcriptome sequencing was performed on NCA-treated and untreated Huh-7 cells to identify differentially expressed genes (DEGs) and signaling pathways. Western blot was used to validate the expressi

SUBMITTER: Xu M 

PROVIDER: S-EPMC12432940 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature