The UBR5 protein facilitates mesangial cell hypertrophy and glycolysis induced by high glucose by increasing the phosphorylation levels of AKT.

ABSTRACT:

Aims

One of the primary pathological features in the early stages of diabetic nephropathy is mesangial cell (MC) hypertrophy in the glomerulus. Considering the role of E3 ubiquitin ligases in regulating MC hypertrophy, the aim of this study was to identify the functional ubiquitin protein ligase E3 component N-recognin 5 (UBR5) during MC hypertrophy under high glucose conditions.

Methods

Human MCs (HMCs) transduced with UBR5 silencing or overexpression vector were treated with high glucose, AKT inhibitor, or glycolysis inhibitor. Cell proliferation, cell cycle, hypertrophy and glycolysis were evaluated in the HMCs after indicated treatment. m6A methylated RNA immunoprecipitation, luciferase reporter assay, and RNA immunoprecipitation were performed to determine the regulatio

SUBMITTER: Liao L 

PROVIDER: S-EPMC12433351 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature