MicroRNA-502-3p Modulates the GABA A Subunits, Synaptic Proteins, and Mitochondrial Morphology in Hippocampal Neurons.

ABSTRACT: MicroRNA-502-3p (MiR-502-3p), a synapse-enriched miRNA, is significantly implicated in Alzheimer's disease (AD). Our previous study revealed a high expression level of miR-502-3p in AD synapses relative to controls. Additionally, miR-502-3p was found to modulate the GABAergic synapse function by modulating the GABA A receptor subunit α-1 (GABRA1) protein. The current study aims to investigate the impact of miR-502-3p on other GABA receptor subunit proteins, synaptic proteins, mitochondrial morphology, and other hippocampal neuron genes. Mouse hippocampal neuronal (HT22) cells were transfected with miR-502-3p overexpression (OE) vector, miR-502-3p suppression (sponge) vector, or scramble control vector. Transfection of miR-502-3p vectors was confirmed by fluorescence microscopy. MiR-502-3p

SUBMITTER: Sharma B 

PROVIDER: S-EPMC12433374 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature