VISTA Alleviates Microglia-Mediated Neuroinflammation After Cerebral Ischemia-Reperfusion Injury via Regulating ACOD1/Itaconic Acid Metabolism.

ABSTRACT: Cerebral ischemia-reperfusion injury (CIRI) induces significant microglial inflammation. V-type immunoglobulin domain-containing suppressor of T cell activation (VISTA), a novel inhibitory immune checkpoint, participates in myeloid cell metabolism. This study aims to investigate the molecular mechanisms of VISTA's protective effects on CIRI by modulating microglial metabolism. In this study, differentially expressed genes (DEGs) were extracted from GSE77986 to identify hub gene VISTA. Transient middle cerebral artery occlusion (tMCAO) and oxygen-glucose deprivation and reoxygenation (OGD/R) were conducted to mimic CIRI. AAVMG1.2-VSIR was injected intracerebroventricularly into Cx3cr1^Cre mice, while over-expression plasmids were transfected into BV2 to intervene VISTA. The mice u

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12433375 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature