Metabolic alterations in diabetic patients: aqueous humor profiling for biomarker discovery.

ABSTRACT: Diabetic retinopathy, a leading cause of vision loss in working-age populations, is a severe complication of diabetes mellitus. Metabolomics, a key approach in systems biology, offers insights into the complex pathophysiology of diabetes by analyzing low-molecular-weight compounds in biological contexts. This study investigated metabolite alterations in the aqueous humor of diabetic and non-diabetic patients undergoing cataract surgery to identify potential biomarkers associated with diabetes. Aqueous humor samples from 191 patients (48 diabetic, 143 non-diabetic) were analyzed using targeted liquid chromatography-mass spectrometry. Metabolite data were normalized and statistically evaluated using univariate analysis, including fold change calculations, t-tests, and volcano plots. Pathway

SUBMITTER: Zemitis A 

PROVIDER: S-EPMC12433443 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature