Rational design of an epitope-centric vaccine against <i>Pseudomonas aeruginosa</i> using pangenomic insights and immunoinformatics approach.

ABSTRACT:

Introduction

As a highly adaptable opportunistic pathogen, Pseudomonas aeruginosa presents a significant threat to people with weakened immune systems. This is because it naturally resists antibiotics and can form biofilms. These factors complicate treatment and underscore the urgent need for innovative therapeutic strategies, such as vaccines, to combat this pathogen.

Methods

A pangenome analysis of P. aeruginosa genomes was performed to identify conserved core genes critical for bacterial survival and virulence. LptF, an outer membrane protein, was prioritized as a target for vaccine development. B-cell and T-cell epitopes were predicted from LptF using immunoinformatics tools, and a multi-epitope peptide vaccine was designed. The interaction between the vacc

SUBMITTER: Mudipalli Elavarasu S 

PROVIDER: S-EPMC12434008 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature